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Spinning Disk Microscopy

Image of Andor revolution

Andor revolution

In contrast to confocal laser scanning microscopes, which have only one pinhole spinning disk microscopes have a disk containing multiple pinholes. This disk is located in the beam path in a conjugate plane to the specimen plane. 
The disk has 20,000 holes arranged in a spiral pattern and this is spun at high speed. As a result of the rapid rotation of the disk at 1800 to 5000 revolutions per minute , a complete image can be created giving the ability to achieve fast frame rates so living processes and dynamic events can be visualised. Resolution and sensitivity however are not as great as for a confocal microscope. 
 
  • High-speed multidimensional imaging with optical sectioning
  • High sensitivity EMCCD camera
  • Laser lines for all classes of fluorescent proteins Incubation for mammalian cells
  • IR reflection based autofocus
  • Incubation chamber and Gas control 
  • Location : Basement Human Biology Building 
  • Contact : Dr. Peter Owens